Sero- and Molecular Epidemiology of HIV-1 in Papua Province, Indonesia

Muhammad Qushai Yunifiar M, Tomohiro Kotaki, Adiana Mutamsari Witaningrum, Siti Qamariyah Khairunisa, Dwi Wahyu Indriati, Meilani Meilani, Tigor Yeheskiel, Shuhei Ueda, Nasronudin Nasronudin, Masanori Kameoka

Abstract


Background: human immunodeficiency virus (HIV) infection and acquired immune deficiency syndrome (AIDS) cause serious health problems and affect the Indonesian economy. Papua province has the highest prevalence of HIV infection in the country; however, epidemiological data are limited. Therefore, in order to reveal the current situation of HIV/AIDS in Papua province, sero- and molecular epidemiological studies of HIV were conducted. Methods: serological tests were conducted on 157 healthy individuals from the general population residing in Paniai, Papua. In addition, a molecular epidemiological study was then conducted on HIV type 1 (HIV-1) genes derived from infected individuals. Peripheral blood samples from HIV-1-positive individuals and 15 additionally enrolled, previously confirmed HIV-1-positive individuals were subjected to a genotypic analysis. Results: serological tests revealed that 2 out of 157 (1.27%) healthy individuals were HIV-positive. In addition, HIV-1 subtyping revealed that subtype B and CRF01_AE were the major subtype and circulating recombinant form (CRF) of HIV-1 prevalent in the region, while subtype A1 and a recombinant form including viral gene fragments of CRF01_AE and subtype B was also detected. In addition, HIV drug resistance-associated major mutations were detected in the reverse transcriptase gene derived from infected individual on antiretroviral therapy. Conclusion: these results provide important information for clearer understanding on the current situation of HIV/AIDS in Papua province in Indonesia.

Keywords


HIV-1; subtype B; CRF01_AE; HIV drug resistance

References


UNAIDS. The Gap report. 2014. [Accessed on 24 February 2017] Available at http://www.unaids.org/sites/default/files/media_asset/UNAIDS_Gap_report_en.pdf

UNAIDS. Epidemiological Fact Sheet on HIV and AIDS (Indonesia). 2013. [Accessed on 24 February 2017] Available at http://www.unaids.org/sites/default/files/epidocuments/IDN.pdf

Ministry of Health of the Republic of Indonesia. Cases of AIDS in Indonesia. 2014. [Accessed on 24 February 2017] Available at http://spiritia.or.id/Stats/StatCurr.php?lang=en

Ministry of Health of the Republic of Indonesia. Risk behavior and HIV prevalence in Tanah Papua 2006. 2006. [Accessed on 24 February 2017] Available at http://siteresources.worldbank.org/INTINDONESIA/Resources/Publication/PapuaHIV_en.pdf

Hemelaar J, Gouws E, Ghys PD, et.al. Global trends in molecular epidemiology of HIV-1 during 2000-2007. AIDS 2011;25:679-689.

Kotaki T, Khairunisa SQ, Sukartiningrum SD, et al. High prevalence of HIV-1 CRF01_AE viruses among female commercial sex workers residing in Surabaya, Indonesia. PLoS One 2013;8:e82645.

Merati TP, Ryan CE, Spelmen T, et al. CRF01_AE dominates the HIV-1 epidemic in Indonesia. Sexual health 2012;9:414-421.

Sahbandar IN, Takahashi K, Djoerban Z, et al. Current HIV type 1 molecular epidemiology profile and identification of unique recombinant forms in Jakarta, Indonesia. AIDS Res Hum Retroviruses 2009;25:637-646.

Sahbandar IN, Takahashi K, Motomura K, et al. The Indonesian variants of CRF33_01B: Near-full length sequence analysis. AIDS Res Hum Retroviruses 2011;27:97-102.

Taylor BS, Sobieszczyk ME, McCutchan FE, et al. The challenge of HIV-1 subtype diversity. N Engl J Med 2008;358:1590-1602.

Tamura K, Stecher G, Peterson D, et al. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 2013;30:2725-2729.

Wensing AM, Calvez V, Gunthard HF, et al. 2015 Update of the drug resistance mutations in HIV-1. Top Antivir Med 2015;23:132-141.

Indonesian National AIDS Commission. Global AIDS response progress reporting: Indonesia country progress report 2014. 2014. [Accessed on 24 February 2017] Available at http://www.unaids.org/sites/default/files/country/documents/IDN_narrative_report_2014.pdf

UNAIDS. HIV transmission in intimate partner relationships in Asia. 2009. [Accessed on 24 February 2017] Available at http://data.unaids.org/pub/Report/2009/intimate_partners_report_en.pdf

Foley B, Donegan E, Silitonga N, et al. Importation of multiple HIV type 1 strains into West Papua, Indonesia (Irian Jaya). AIDS Res Hum Retroviruses 2001;17:1655-1659.

Witaningrum AM, Kotaki T, Khairunisa SQ, et al. Genotypic characterization of human immunodeficiency virus type 1 derived from antiretroviral therapy-naive individuals residing in Sorong, West Papua. AIDS Res Hum Retroviruses 2016;32:812-817.

Khairunisa SQ, Kotaki T, Witaningrum AM, et al. Appearance of Drug Resistance-Associated Mutations in Human Immunodeficiency Virus Type 1 Protease and Reverse Transcriptase Derived from Drug-Treated Indonesian Patients. Aids Research And Human Retroviruses 2015;31:255-259.


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