Phenotype Characterization of Beta-Lactamase Producing Enterobacteriaceae in the Intensive Care Unit (ICU) of Cipto Mangunkusumo Hospital in 2011

Authors

  • Yulia R Saharman Department of Microbiology, Faculty of Medicine, University of Indonesia – Cipto Mangunkusumo Hospital
  • Delly C Lestari Department of Microbiology, Faculty of Medicine, University of Indonesia – Cipto Mangunkusumo Hospital

Abstract

Aim: the goal of this study is to understand the phenotype characteristic of beta-lactamase enzymes producing Enterobacteriaceae, such as ESBL, AmpC, and carbapenemase. Methods: three different methods are performed to confirm those phenotypic characteristics, namely double disk diffusion method to confirm ESBL, AmpC disk test (cefoxitin-based) to confirm AmpC, and modified Hodge test to confirm carbapenemase. Results: using double disk diffusion method, we found 58.42% isolates are ESBL-producing, whereas the outcomes of AmpC disk test shows 1.98% are AmpC-producing. By conducting modified Hodge test (MHT), 27.59% isolates are confirmed as carbapenemase-producing bacteria. Conclusion: this study confirmed the prevalence of beta-lactamase producing Klebsiella pneumoniae is very high. Nevertheless, AmpC and carbapenemase should not be ignored despite their low prevalence. Key words: Enterobacteriaceae, antibiotic resistance, ESBL, AmpC, carbapenemase.

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Published

2016-05-18

How to Cite

Saharman, Y. R., & Lestari, D. C. (2016). Phenotype Characterization of Beta-Lactamase Producing Enterobacteriaceae in the Intensive Care Unit (ICU) of Cipto Mangunkusumo Hospital in 2011. Acta Medica Indonesiana, 45(1). Retrieved from https://actamedindones.org/index.php/ijim/article/view/115

Issue

Vol. 45 No. 1 (2013)

Section

ORIGINAL ARTICLE

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