Induction of Rat Germ Cell Apoptosis by Testosterone Undecanoate and Depot Medroxyprogesterone Acetate and Correlation of Apoptotic Cells with Sperm Concentration

Authors

  • Syafruddin Ilyas Department of Biology, Faculty of Mathematics and Natural Sciences, University of North Sumatra. Indonesia
  • Silvia W Lestari Department of Medical Biology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
  • Nukman Moeloek Department of Medical Biology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
  • Asmarinah Asmarinah Department of Medical Biology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
  • Siregar C Siregar Department of Anatomy and Pathology, Faculty of Medicine, University of Indonesia, Jakarta, Indone

Abstract

Aim: to investigate the safety and effectiveness of dose TU+DMPA hormones in developing potential male contraceptives. Methods: suppression of rat sperm concentration through increased apoptotic germ cells by in vivo administration of a long-acting androgen composed of a combination of testosterone undecanoate (TU) and depot medroxyprogesterone acetate (DMPA) was performed. Adult Sprague-Dawley rats received 2.5 mg TU every 6 week and 1.25 mg DMPA every 12 week for 60 week, a regimen known to rapidly reduce testosterone production by the testes and produce azoospermia within 12 week. Sperm concentration data were log transformed before analysis. Results are expressed as the mean±SEM. ANOVA, followed by post hoc test was used to determine differences across time and phase. T test was employed to determine differences between two groups. Results: apoptosis revealed significant increase in apoptotic germ cells (80% when rats were administered with TU+DMPA. Apoptotic germ cells can be found in several spermatogonia (20%), spermatocytes (30%), and spermatids (50%). Conclusion: dose TU+DMPA hormones may be a safe and effective way to develop potential male contraceptives. Key words: TU, DMPA, sperm concentration, apoptosis.

Downloads

Published

2016-05-18

How to Cite

Ilyas, S., Lestari, S. W., Moeloek, N., Asmarinah, A., & Siregar, S. C. (2016). Induction of Rat Germ Cell Apoptosis by Testosterone Undecanoate and Depot Medroxyprogesterone Acetate and Correlation of Apoptotic Cells with Sperm Concentration. Acta Medica Indonesiana, 45(1). Retrieved from https://actamedindones.org/index.php/ijim/article/view/118

Issue

Vol. 45 No. 1 (2013)

Section

ORIGINAL ARTICLE

License

Copyright

The authors who publish in this journal agree to the following requirements:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a  Creative Commons Attribution 4.0 International License (CC BY 4.0) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors can enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) before and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work. (See The Effect of Open Access)

Privacy Statement

The names and email addresses entered in this journal site will be used exclusively for the stated purposes of this journal and will not be made available for any other purpose or to any other party.