Level of Circulating Endothelial Cells and Expression of Nuclear Factor Kappa Beta of Human’s Peripheral Blood Mononuclear Cells in Subjects with Certain Conditions

Authors

  • Titin A Wihastuti Department of Biomedicine, Faculty of Medicine, Brawijaya University, Malang, Indonesia
  • Djanggan Sargowo Department of Cardiology and Vascular Medicine, Faculty of Medicine, Brawijaya University, Malang, Indonesia
  • Achdiat Agoes Department of Cardiology and Vascular Medicine, Faculty of Medicine, Brawijaya University, Malang, Indonesia
  • Satuman Satuman Department of Cardiology and Vascular Medicine, Faculty of Medicine, Brawijaya University, Malang, Indonesia
  • Teuku Heriansyah Faculty of Medicine, Syiah Kuala University, Aceh, Indonesia

Abstract

Aim: to detect the levels of CEC and expression of NFκB in the three groups of subjects with certain conditions. Methods: this study is an exploratory study using human peripheral blood samples. The study subjects comprised three groups, the group of 23 healthy people, a group of 35 people that has one or more risk factors for vascular disease and a group of 15 vascular disease patients (coronary heart disease, diabetes mellitus, stroke). CEC were isolated from peripheral blood mononuclear cells (PBMC). CEC level is identified through the measurement of CD45 and CD146 by flowcytometry method. NFκB expression is recognized by ELISA method (imgenex, USA). Results: the highest average levels of CEC were found in the sick group (28.6%). The highest average expression of NFκB (924.9495) is found in the group with risk factors. The lowest average expression of NFκB and CEC is found in the healthy group. Statistical analysis of ANOVA at the interval confidence of 95% shows a significant difference (p=0.00) levels of CEC and NFκB expression between the healthy group with the group with the risk of cardiovascular disease (CVD) and patients with known CVD. Conclusion: increase of level CEC and NFκB expression has a strong relationship with vascular disease and its risk factors. Key words: circulating endothelial cells (CEC), nuclear factor kappa beta (NFκB), peripheral blood mononuclear cell (PBMC), vascular disease.

Downloads

Published

2014-01-01

How to Cite

Wihastuti, T. A., Sargowo, D., Agoes, A., Satuman, S., & Heriansyah, T. (2014). Level of Circulating Endothelial Cells and Expression of Nuclear Factor Kappa Beta of Human’s Peripheral Blood Mononuclear Cells in Subjects with Certain Conditions. Acta Medica Indonesiana, 46(1). Retrieved from https://actamedindones.org/index.php/ijim/article/view/61

Issue

Vol. 46 No. 1 (2014)

Section

ORIGINAL ARTICLE

License

Copyright

The authors who publish in this journal agree to the following requirements:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a  Creative Commons Attribution 4.0 International License (CC BY 4.0) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors can enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) before and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work. (See The Effect of Open Access)

Privacy Statement

The names and email addresses entered in this journal site will be used exclusively for the stated purposes of this journal and will not be made available for any other purpose or to any other party.