Profile of PRMT-1 Gene Polymorphism in Hemodialysis Patients with Increased ADMA Levels

Authors

  • Mochammad Thaha Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga - Dr. Soetomo Hospital, Surabaya, Indonesia
  • Wenny P Nilamsari Department of Clinical Pharmacy, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia
  • Mochammad Yusuf Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Airlangga - Dr Soetomo Hospital, Surabaya, Indonesia
  • M. Amin Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia

Abstract

Aim: to determine the distribution of PRMT-1 gene polymorphism and ADMA levels among continuing hemodialysis patients. Methods: genotyping of PRMT-1 polymorphism was performed in 57 hemodialysis patients at Al Irsjad Hospital. All participants were recruited for physical examination, questionnaire, and collection of 5 mL fasting venous blood. The blood was treated with phenol-chloroform extraction of genomic DNA. The candidate’s single nucleotide polymorphisms (SNPs) were genotyped by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The ADMA plasma levels was determined by ELISA and all biochemical indicators of serum were examined. Results: fifty-seven hemodialysis patients participated in our study, 54 (95.4%) of them had increased ADMA plasma levels. DNA sequencing analysis of 13 patients showed a suspected PRMT-1 gene polymorphism at sequence 5837 as there were different genotypes between C and G. Conclusion: the increased levels of ADMA might be caused by PRMT-1 gene polymorphism.Key words: ADMA, PRMT-1 polymorphism, chronic kidney disease

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Published

2016-05-16

How to Cite

Thaha, M., Nilamsari, W. P., Yusuf, M., & Amin, M. (2016). Profile of PRMT-1 Gene Polymorphism in Hemodialysis Patients with Increased ADMA Levels. Acta Medica Indonesiana, 46(2). Retrieved from https://actamedindones.org/index.php/ijim/article/view/74

Issue

Vol. 46 No. 2 (2014)

Section

ORIGINAL ARTICLE

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