1. Home
  2. Vol 54, No 4 (2022)
  3. Vidyani

Lower Number and Percentage of Activated Natural Killer Cells in Colorectal Cancer Patients

Amie Vidyani, Iswan Abbas Nusi, Ulfa Kholili, Poernomo B Setiawan, Herry Purbayu, Titong Sugihartono, Ummi Maimunah, Budi Widodo, Husin Thamrin, Muhammad Miftahussurur

Abstract


Background: Colorectal cancer is a type of cancer that begins in the colon and/or rectum tissue. Natural killer (NK) cells play a critical role in the first line of defense against infection and tumors, as well as in autoimmunity and hypersensitivity reactions. NK cells also play a role in regulating tumor cell growth and metastasis. The number and percentage of activated natural killer cells have been determined in patients with colorectal cancer and benign lesion. Methods: This was a cross-sectional observational analytic study. The number and percentage of activated NK cells in peripheral blood were determined using the flow cytometry method in 50 samples from patients who underwent colonoscopy and obtained a mass as evidenced by histopathological examination. Results: Among the 50 samples, 24 samples included in the colorectal cancer group and 26 samples from benign lesion group. The mean number of NK cells in colorectal cancer was 161.71 ± 62.666 cells/µL, benign lesion was 553.92 ± 269.173 cells/µL. The mean percentage of activated NK cells in colorectal cancer was 2.82 ± 1.19%, benign lesion was 5.10 ± 2.48%. There was a significant difference in the number of NK cells and the percentage of activated NK cells between colorectal cancer and benign lesion patients (p = 0.000). Conclusion: The number and activity of NK cells decreases in patients with colorectal cancer.

Keywords


The number of NK cells; percentage of activated NK cells; colorectal cancer; cancer

References


Granados-Romero JJ, Valderrama-Treviño AI, Contreras-Flores EH, et al. Colorectal cancer: a review. Int J Res Med Sci. 2017;5(11):4667.

Gandomani HS, Yousefi SM, Aghajani M, et al. Colorectal cancer in the world: incidence, mortality and risk factors. Biomed Res Ther. 2017;4(10):1656.

Schreuders EH, Ruco A, Rabeneck L, et al. Colorectal cancer screening: a global overview of existing programmes. Gut. 2015;64(10):1637–49.

Paul S, Lal G. The molecular mechanism of natural killer cells function and its importance in cancer immunotherapy. Front Immunol. 2017;8.

Tang YP, Xie MZ, Li KZ, Li JL, Cai ZM, Hu BL. Prognostic value of peripheral blood natural killer cells in colorectal cancer. BMC Gastroenterol. 2020;20(1):1–11.

Jobin G, Rodriguez-Suarez R, Betito K. Association between natural killer cell activity and colorectal cancer in high-risk subjects undergoing colonoscopy. Gastroenterol. 2017;153(4):980–7. Available from: http://dx.doi.org/10.1053/j.gastro.2017.06.009

Mordoh J, Levy EM, Roberti MP. Natural killer cells in human cancer: From biological functions to clinical applications. J Biomed Biotechnol. 2011;2011.

Sconocchia G, Eppenberger S, Spagnoli GC, et al. Nk cells and T cells cooperate during the clinical course of colorectal cancer. Oncoimmunology. 2014;3(8):1–6.

Desbois M, Rusakiewicz S, Locher C, Zitvogel L, Chaput N. Natural killer cells in non-hematopoietic malignancies. Front Immunol. 2012;3:1–12.

Dozois EJ, Boardman LA, Suwanthanma W, et al. Young-onset colorectal cancer in patients with no known genetic predisposition: Can we increase early recognition and improve outcome? Medicine (Baltimore). 2008;87(5):259–63.

Khosama Y. Faktor risiko kanker kolorektal. Cermin Dunia Kedokt. 2015;42(11):829–32.

Binefa G, Rodríguez-Moranta F, Teule À, Medina-Hayas M. Colorectal cancer: From prevention to personalized medicine. World J Gastroenterol. 2014;20(22):6786–808.

Lin JH, Zhang SM, Rexrode KM, et al. Association between sex hormones and colorectal cancer risk in men and women. Clin Gastroenterol Hepatol. 2013;11(4):419–24.

Johnson IT, Lund EK. Review article: Nutrition, obesity and colorectal cancer. Aliment Pharmacol Ther. 2007;26(2):161–81.

Sudoyo AW, Hernowo B, Krisnuhoni E, Reksodiputro AH, Hardjodisastro D, Sinuraya ES. Colorectal cancer among young native indonesians: A clinicopathological and molecular assessment on microsatellite instability. Med J Indones. 2010;19(4):245–51.

Ollberding NJ, Nomura AMY, Wilkens LR, Henderson BE, Kolonel LN. Racial/ethnic differences in colorectal cancer risk: the multiethnic cohort study. Int J cancer. 2011;129(8):1899–906.

Yunusova NV, Stakheyeva MN, Molchanov SV, et al. Functional activity of natural killer cells in biological fluids in patients with colorectal and ovarian cancers. Cent Eur J Immunol. 2018;43(1):26–32.

Yamaguchi M, Okamura S, Yamaji T, et al. Plasma cytokine levels and the presence of colorectal cancer. PLoS One. 2019;14(3):1–13.

Leibson PJ. Signal transduction during natural killer cell activation: Inside the mind of a killer. Immunity. 1997;6(6):655–61.

Shen Y, Wang Q, Qi Y, et al. Peripheral Foxp3+ regulatory T cells and natural killer group 2, member D expression levels in natural killer cells of patients with colorectal cancer. Mol Med Rep. 2014;10(2):977–82.

Shen Y, Lu C, Tian W, Wang L, Cui B, Jiao Y, et al. Possible association of decreased NKG2D expression levels and suppression of the activity of natural killer cells in patients with colorectal cancer. Int J Oncol. 2012;40(4):1285–9.


Full Text: PDF

Refbacks

  • There are currently no refbacks.